Week 1- Day 1 30th of June 2014

Darwin-The beginning of Phylogeny.

Darwin-The beginning of Phylogeny.

Really enjoying the lab!

Really enjoying the lab!

 

Claire busy with Dr.Lao.

Claire busy with Dr.Lao.

Amazing to be hands on already!

Amazing to be hands on already!

 

  • Absolutely thrilled to be involved in this summer science programme. Many thanks to Professor Teeling, Dr. Joanna Kacprzyck and all in the UCD Bat Lab for the opportunity and their time.
  • I am so looking forward to learning! Yes I can’t wait to brush up on those lab skills I last used over 17 years ago and to experience life in the world of research. It can be daunting to immerse yourself into new professional experiences and Yes I am a little apprehensive but I know that I will gain so much and hopefully contribute as well.
  • Dr. Joanna Kacprzyck welcomes myself and my partner for the month Clare Lamont to the lab. The bat lab is a hive of activity, half the team are heading to France for field studies and the preparations are paramount to a successful outcome. Bon Voyage et Bon Chance!
  • Clare and I meet Dr. Nga Lao,our tutor for the week and someone with a wealth of experience in research.
  • Apprehensive at this stage-Can I remember those lab skills? Will I be able to absorb all these details and protocols? There is no need to worry-it’s amazing what comes back to you. So if you are reading this and considering a summer science programme-do it! Your skills will come flooding back and if you are from a different speciality you will receive the best tutorage! Many thanks Dr.Lao.
  • Dr.Lao helps Clare and I get familiar with some key pieces of equipment- we will be extracting and purifying DNA using the liquid-liquid extraction method.
  • Leaving Cert Biology-we extract DNA from onions or fruit and if it works well the student is able to scoop some DNA out of the test tube. What you are scooping out is DNA but it contains many other molecules and is not very pure and the volume is enormous as compared to what you would extract in the bat lab.
  • Dr. Lao taught Clare and I the phenol-chloroform liquid-liquid extraction method.
  • DNA extracted from tiny bat blood samples is extracted and purified using chloroform, phenol and sodium hydroxide. These solutions are added to the samples, these are then centrifuged (spun at high speeds) and the DNA will become trapped in the upper liquid layer of the sample. This is removed and the DNA is purified further by alcohol precipitation.
  • The lab work was amazing and it was fantastic to be immediately immersed in the practicals.
  • When you experience the other side of science you really do become reinvigorated-I already have ideas for junior and senior science.
  • Clare and I have already discussed our TY programme-planning it already!
  • Great Day!
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