Friday has arrived-Last day of Gel electrophoresis before we embark on bioinformatics.
Samples that underwent PCR last night are now ready to be visualised.
You by now know the routine-prepare the gel- let it set. Get your samples, the positive and negative controls and the DNA ladder. Add 2ul of the UV safe dye into your wells and then add the volume of sample you have decided on to the wells with the dye.
Place the gel in the buffer and load the lanes of the gel carefully. Run the electricity for 20 mins. Remove gel and place in the UV chamber and run the programme. DNA barcode is visualised on the screen.
Unfortunately our winning streak abandoned us this morning- quality was not great and so we decided to repeat the electrophoresis again. Just waiting now for the gel to set, will let you know how we get on.
Afternoon DNA barcode looks great. Electrophoresis worked really well.
First week ends on a high, it’s amazing how much I have learned in terms of lab work and genetic theory. Thanks to all at the Bat lab and special thanks to Dr.Lao.