Week 1- Day 5

Look for the mitochondria-Mitichondrial DNA is most often used in evolutionary genetics- Cytochrome b is the gene we are working with.

Look for the mitochondria-Mitichondrial DNA is most often used in evolutionary genetics- Cytochrome b is the gene we are working with.

Friday has arrived-Last day of Gel electrophoresis before we embark on bioinformatics.

Samples that underwent PCR last night are now ready to be visualised.

You by now know the routine-prepare the gel- let it set. Get your samples, the positive and negative controls and the DNA ladder. Add 2ul of the UV safe dye into your wells and then add the volume of sample you have decided on to the wells with the dye.

Place the gel in the buffer and load the lanes of the gel carefully. Run the electricity for 20 mins. Remove gel and place in the UV chamber and run the programme. DNA barcode is visualised on the screen.

Unfortunately our winning streak abandoned us this morning- quality was not great and so we decided to repeat the electrophoresis again. Just waiting now for the gel to set, will let you know how we get on.

image

1% Agarose Gel Left to set.

Afternoon DNA barcode looks great. Electrophoresis worked really well.

DNA band very visible in third lane from the left. Nothing in the negative control which is very important. Clare's results are in top lanes, mine are in the bottom lanes.

DNA band very visible in third lane from the left. Nothing in the negative control which is very important. Clare’s results are in top lanes, mine are in the bottom lanes.

First week ends on a high, it’s amazing how much I have learned in terms of lab work and genetic theory. Thanks to all at the Bat lab and special thanks to Dr.Lao.

Advertisements

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s